https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:33857 Setaria viridis and its close domesticated relative Setaria italica have been chosen as suitable monocotyledonous models for plants possessing the C₄ pathway of photosynthesis including sorghum, maize, sugarcane, switchgrass and Miscanthus×giganteus. Accurate partial least squares regression (PLSR) models to predict S. italica stem composition have been generated, based upon Fourier transform mid-infrared (FTIR) spectra and calibrated with wet chemistry determinations of ground S. italica stem material measured using a modified version of the US National Renewable Energy Laboratory (NREL) acid hydrolysis protocol. The models facilitated a high-throughput screening analysis for glucan, xylan, Klason lignin and acid soluble lignin (ASL) in a collection of 183 natural S. italica variants and clustered them into classes, some possessing unique chemotypes. The predictive models provide a highly efficient screening tool for large scale breeding programs aimed at identifying lines or mutants possessing unique cell wall chemotypes. Genes encoding key catalytic enzymes of the lignin biosynthesis pathway exhibit a high level of conservation with matching expression profiles, measured by RT-qPCR, among accessions of S. italica, which closely mirror profiles observed in the different developmental regions of an elongating internode of S. viridis by RNASeq.]]> Thu 21 Oct 2021 12:52:27 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:31195 in vitro analysis was conducted, whereby changes in pH and lactic acid metabolism were compared in identical acidified rumen samples, following inoculation with various propionibacteria. This was followed by a study to evaluate the effect of bacterial inoculation dosage on acid metabolism. The results indicated that lactic acid levels in the rumen fluid were significantly reduced, and propionic acid and acetic acid concentrations both significantly increased, following addition of propionibacteria. Significant ‘between strains’ differences were observed, with Propionibacterium acidopropionici 341, Propionibacterium freudenreichii CSCC 2207, Propionibacterium jensenii NCFB 572 and P. jensenii 702 each producing more rapid reduction of lactic acid concentration than P. freudenreichii CSCC 2206, P. acidopropionici ATCC 25562 and Propionibacterium thoenii ATCC 4874. Furthermore, the efficacy of this application was dosage related, with the rates of reduction in lactic acid levels and production of propionic acid, both significantly greater for the higher (10¹⁰ cfu mL⁻¹) compared with lower (10⁵ cfu mL⁻¹) dosage inoculation. The results confirmed that the introduction of propionibacteria could promote more rapid reduction of lactic acid levels than would occur without their addition, demonstrating their potential in controlling ruminal acidosis.]]> Sat 24 Mar 2018 08:44:46 AEDT ]]>